Micro Cuttings Propagation Of Pıtcher Plant (Nephenthes spp) On Various Media And Indole Acetıc Acıd (IAA) By In Vitro

Abstract

Pitcher plants is a unique type of ornamental plant and is one of the typical insectivorous plants of the tropics. Propagation of pitcher ornamental plants can be done through in vitro culture using young plant parts such as young leaves and stems. The study aimed to examine the interaction between media types and NAA concentrations to obtain the right media types and IAA concentrations to support the growth of pitcher plants micro-cuttings in vitro. Research is a laboratory experiment arranged in a Complete Randomized Design with two factors. The first factor is the treatment of various media, namely MS media, MS media with vitamin B5, and 1/2 MS media with vitamin B5. The second factor is the IAA concentration, which is 0.5 ppm, 1 ppm, and 1.5 ppm. The observed data were analyzed with ANOVA (Analysis of Variant) at the real level of 5% and further tested with Duncan's Multiple Range Test (DMRT) at the level of 5%. The results showed an interaction between MS media treatment and an IAA concentration of 1 ppm on the height parameter of the plantlet. Using MS media and MS media with vitamin B5 gives good results on the parameters when shoots appear, the number of shoots, and leaves. IAA 0.5 ppm and 1 ppm give the best results on the parameters at the emergence of shoots and the number of bags of nepenthes.